These web pages present the videos recorded during a live-imaging screen in HEK293 cells. This was aimed at identifying novel cytoplasmic proteins implicated in signaling pathways of G-protein coupled receptors, based on a translocation criterion (1).
The plasmids transfected in cells during the screen were from the GFP-tagged human full-length ORF plasmid collection and encoded proteins that localize in the cytoplasm (2-3). This plasmid library is composed of two vectors for each cDNA, one with the cDNA encoding CFP in frame 5’ of the ORF, the other with the cDNA encoding YFP in frame 3’ of the ORF.
An extensive study of REDD1, one of the hit proteins can be found in (4).
To get access to the list of the screened proteins, press « Show all ».
To refine the Search, use keywords such as : Name, full-Name, cDNA ID, Swissprot ID, biological process.
Id | Name | Full Name | Biological Process | Clones | cDNA ID | Swissprot ID | Endogenous localisation in Stadler et al. 2013 | Fusion Orientation | Localisation in Simpson et al. 2000 | Localisation during screening | Comments during screening |
---|---|---|---|---|---|---|---|---|---|---|---|
289 | WDR91 | WD repeat-containing protein 91 | unknown | hute1_24b4 | AL136940 | Q9H062 | IF cyt/nuc not nucleoli/ Golgi | CFP-ORF | cyt | cyt | |
290 | WDR91 | WD repeat-containing protein 91 | unknown | hute1_24b4 | AL136940 | Q9H062 | IF cyt/nuc not nucleoli/ Golgi | ORF-YFP | cyt/ves | cyt + PM sometimes +vesicles |